MHC Class II I-Ek 抗体
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北京 101111
Quick Overview for MHC Class II I-Ek 抗体 (ABIN114319)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G chromatography
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免疫原
- C3H Donor: C3H.SW Fusion Partner: SP2/0-Ag 14
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亚型
- IgG2a
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anti-MHC Class II I-Ek antibody (Biotin)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S Biotin
anti-MHC Class II I-Ek antibody (PE)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S PE
anti-MHC Class II I-Ek antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S FITC
anti-MHC Class II I-Ek antibody适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 17-3-3S unconjugated
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应用备注
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Cytotoxicity analysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 1000 and mix.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS and 0.02 % NaN3
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II I-Ek
抗原
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