MHC Class II I-Ap 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS 和 CyTox. Suitable for 小鼠.
Quick Overview for MHC Class II I-Ap 抗体 (ABIN114314)
抗原
MHC Class II I-Ap
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Cytotoxicity Test (CyTox)
克隆位点
7-16-17
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纯化方法
- Protein G chromatography
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免疫原
- B10.p
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亚型
- IgG2a
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应用备注
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Flow cytometry (protocol see below). Cytotoxic assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS and 0.02 % sodium azide (NaN3).
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II I-Ap
抗原
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