MHC Class II I-Ap 抗体
The 小鼠 单克隆 anti-MHC Class II I-Ap antibody is suitable to detect MHC Class II I-Ap in samples from 小鼠. It has been validated for FACS 和 CyTox.
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产品编号 ABIN114311
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MHC Class II I-Ap 抗体 (ABIN114311)
抗原
MHC Class II I-Ap
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Cytotoxicity Test (CyTox)
克隆位点
7-16-17
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纯化方法
- Ig Fraction
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免疫原
- Recipient: BALB/c Donor: B10.P Fusion Partner: Spleen from immunized recipient fused with myeloma SP2/0
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亚型
- IgG2a
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应用备注
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Cytotoxicity analysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF I-A ANTIGEN BEARINGCELLS: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium®1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte-M®2 density cell separation medium. After washing, adjustthe cell concentration to 1 x 10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 40 and mix. Alternatively, pellet the cellsand resuspend in antibody diluted 1: 40 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 1.0 mL of distilled water.
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缓冲液
- PBS
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注意事项
- Use of sodium azide as a preservative will substantially inhibit the enUse of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
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注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II I-Ap
抗原
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