MHC Class I (H-2Kb/H-2Db) 抗体 (FITC)
This 小鼠 单克隆 antibody specifically detects MHC Class I (H-2Kb/H-2Db) in FACS. It exhibits reactivity toward 小鼠.
产品编号 ABIN114295
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MHC Class I (H-2Kb/H-2Db) 抗体 (FITC) (ABIN114295)
抗原
MHC Class I (H-2Kb/H-2Db)
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
FITC
应用范围
Flow Cytometry (FACS)
克隆位点
5041-16-1
-
-
纯化方法
- Protein G Chromatography
-
亚型
- IgG2a
-
-
-
-
应用备注
-
Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube add 0. 1 mg of antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold Media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in phosphate buffered saline. (This stains dead cells by intercalatingDNA. )
-
限制
- 仅限研究用
-
-
-
浓度
- 0.1 mg/mL
-
缓冲液
- PBS with the addition of 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
-
有效期
- 6 months
-
-
- MHC Class I (H-2Kb/H-2Db)
-
别名
- MHC Class I H-2 Kb / H-2 Db
-
背景
- Synonyms: HLA Class I
抗原
-
You are here:
