MHC Class I (H-2Kb/H-2Db) 抗体 (Biotin)
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Quick Overview for MHC Class I (H-2Kb/H-2Db) 抗体 (Biotin) (ABIN114294)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Affinity Chromatography on Protein A.
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亚型
- IgG2a
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应用备注
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Flow Cytometry (see Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSISMethod: 1. Prepare a cell suspension in media A. For spleen cell preparations, deplete the red blood
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限制
- 仅限研究用
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浓度
- 0.1 mg IgG /ml
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缓冲液
- PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class I (H-2Kb/H-2Db)
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别名
- MHC Class I H-2 Kb / H-2 Db
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背景
- Synonyms: HLA Class I
抗原
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