MHC Class I (H-2Kb/H-2Db) 抗体
The 小鼠 单克隆 anti-MHC Class I (H-2Kb/H-2Db) antibody is suitable to detect MHC Class I (H-2Kb/H-2Db) in samples from 小鼠. It has been validated for CyTox.
1 image
产品编号 ABIN114293
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MHC Class I (H-2Kb/H-2Db) 抗体 (ABIN114293)
抗原
MHC Class I (H-2Kb/H-2Db)
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Cytotoxicity Test (CyTox)
克隆位点
5041-16-1
-
-
纯化方法
- Protein G Chromatography
-
亚型
- IgG2a
-
-
-
-
应用备注
-
Cytotoxicity assays. Functional testing. (For details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF H-2Kb AND/OR H-2DbBEARING LYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in a cytotoxicity medium (1) orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on a density cell separation medium (2). After washing, adjust the cell concentration to 1x10e7 cells per ml in cytotoxicity medium. 2. Add the antibody to a final concentration of 1: 100 and mix. Alternatively, pellet the cellsand resuspend in antibody diluted 1: 100 in cytotoxicity medium. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant5. Resuspend to the original volume in a low-toxicity rabbit complement (3), diluted to theappropriate concentration in cytotoxicity medium. 6. Incubate for 60 minutes at 37°C. 7. Monitor for percent cytotoxicity at this stage, before further processing. For this purpose
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- PBS, without preservative
-
储存液
- Without preservative
-
注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- MHC Class I (H-2Kb/H-2Db)
-
别名
- MHC Class I H-2 Kb / H-2 Db
-
背景
- Synonyms: HLA Class I
抗原
-
You are here:
