H-2Dd 抗体
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北京 101111
Quick Overview for H-2Dd 抗体 (ABIN114286)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This mAb is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate or eliminate cells bearing H-2Dd (H-2.4) antigen from the appropriate strains of mice.
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recipient: C3H/HeJ Donor: B6XDBA/2 spleen cells Fusion Partner: SP2/0.Ag14
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亚型
- IgG2a
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anti-MHC Class I H-2Dd antibody (Biotin)
适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S Biotin
anti-MHC Class I H-2Dd antibody (PE)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S PE
anti-MHC Class I H-2Dd antibody适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S unconjugated
anti-MHC Class I H-2Dd antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S FITC
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应用备注
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Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OFH-2Dd BEARING LYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viable
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, no preservative.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- H-2Dd (MHC Class I H-2Dd)
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别名
- MHC Class I H-2 Dd
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背景
- Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1
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基因ID
- 100045864
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NCBI登录号
- XP_003086970
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UniProt
- P01900
抗原
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