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- 抗原 See all CD90 (THY1) 抗体
- CD90 (THY1)
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD90 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
- 纯化方法
- Protein G Chromatography
- 免疫原
- CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
- 克隆位点
- 5a-8
- 亚型
- IgG2b
- Top Product
- Discover our top product THY1 Primary Antibody
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anti-CD90 (THY1) (AA 31-120) antibody
THY1 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (AA 17-132) antibodyTHY1 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7E1B11 unconjugated
anti-CD90 (THY1) (AA 20-133) antibodyTHY1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (AA 36-65), (N-Term) antibodyTHY1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03970 unconjugated
anti-CD90 (THY1) (AA 1-161) antibodyTHY1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F9 unconjugated
anti-CD90 (THY1) antibodyTHY1 适用: 人, 猴, Baboon FACS, IHC, IHC (fro), IP, IHC (p) 宿主: 小鼠 Monoclonal 5E10 unconjugated
anti-CD90 (THY1) (AA 20-131) antibodyTHY1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (Internal Region) antibodyTHY1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) antibody (FITC)THY1 适用: 人, Pig, 马, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 5E10 FITC
anti-CD90 (THY1) antibodyTHY1 适用: 小鼠 FACS, CyTox 宿主: 小鼠 Monoclonal F7D5 unconjugated
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- 应用备注
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Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: PE-Mouse IgG2b,kCell Source Percentage of cells stained above control: Thymus: 98. 4%Spleen: 28. 8%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR/J
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- 抗原
- CD90 (THY1)
- 别名
- CD90 (THY1 产品)
- 背景
- CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
- 基因ID
- 21838
- NCBI登录号
- NP_033408
- UniProt
- P01831
- 途径
- Cell-Cell Junction Organization
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