CD90 抗体
Quick Overview for CD90 抗体 (ABIN114254)
抗原
See all CD90 (THY1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
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纯化方法
- Purified
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免疫原
- CBA/J
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亚型
- IgG2b
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anti-CD90 (THY1) (AA 31-120) antibody
THY1 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (AA 17-132) antibodyTHY1 适用: 人 WB, IHC, ICC, ELISA 宿主: 小鼠 Monoclonal 7E1B11 unconjugated
anti-CD90 (THY1) (AA 20-133) antibodyTHY1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (AA 36-65), (N-Term) antibodyTHY1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03970 unconjugated
anti-CD90 (THY1) antibodyTHY1 适用: 人, 猴, Baboon FACS, IHC, IP, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 5E10 unconjugated
anti-CD90 (THY1) (AA 1-161) antibodyTHY1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F9 unconjugated
anti-CD90 (THY1) (AA 20-131) antibodyTHY1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (Internal Region) antibodyTHY1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) antibody (FITC)THY1 适用: 人, Pig, 马, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 5E10 FITC
anti-CD90 (THY1) antibodyTHY1 适用: 小鼠 FACS, CyTox 宿主: 小鼠 Monoclonal F7D5 unconjugated
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应用备注
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Immunohistochemistry on frozen sections. Cytotoxicity Analysis (see protocol).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells,representing 1 test). 4. To each tube, add 1. 0 ug of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that tubes are protected from light since most fluorochromes are lightsensitive)7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Mouse Strain: BALB/cCell Concentration : 1x10e6 cells per testAntibody Concentration Used: 1. 0 ug / 10e6 cellsIsotypic Control: APC Mouse IgG2bCell Source-Percentage of cells stained above control: Thymus: 99. 8%Strain Distribution: Tissue: ThymusCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 ug/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATL
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.02 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- CD90 (THY1)
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别名
- CD90
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背景
- CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
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基因ID
- 21838
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NCBI登录号
- NP_033408
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UniProt
- P01831
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途径
- Cell-Cell Junction Organization
抗原
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