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- 抗原 See all CD90 (THY1) 抗体
- CD90 (THY1)
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD90 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
- 纯化方法
- Protein G Chromatography
- 免疫原
- CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
- 克隆位点
- 5a-8
- 亚型
- IgG2b
- Top Product
- Discover our top product THY1 Primary Antibody
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anti-CD90 (THY1) (AA 31-120) antibody
THY1 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (AA 17-132) antibodyTHY1 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7E1B11 unconjugated
anti-CD90 (THY1) (AA 20-133) antibodyTHY1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (AA 36-65), (N-Term) antibodyTHY1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03970 unconjugated
anti-CD90 (THY1) (AA 1-161) antibodyTHY1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3F9 unconjugated
anti-CD90 (THY1) antibodyTHY1 适用: 人, 猴, Baboon FACS, IHC, IHC (fro), IP, IHC (p) 宿主: 小鼠 Monoclonal 5E10 unconjugated
anti-CD90 (THY1) (AA 20-131) antibodyTHY1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) (Internal Region) antibodyTHY1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD90 (THY1) antibody (FITC)THY1 适用: 人, Pig, 马, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 5E10 FITC
anti-CD90 (THY1) antibodyTHY1 适用: 小鼠 FACS, CyTox 宿主: 小鼠 Monoclonal F7D5 unconjugated
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- 应用备注
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Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1/50 -1/100 dilution in 50 µl of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive)7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1/50 in 50 µl/10e6 cellsIsotypic Control: FITC Mouse IgG2bCell Source Percentage of cells stained above control: Thymus: 97. 4%Spleen: 52. 6%Results - Strain Distribution: Tissue: SpleenCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1/100 in 50 µl/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD90 (THY1)
- 别名
- CD90 (THY1 产品)
- 背景
- CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
- 基因ID
- 21838
- NCBI登录号
- NP_033408
- UniProt
- P01831
- 途径
- Cell-Cell Junction Organization
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