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CD90 抗体 (Biotin)

THY1 适用: 小鼠 FACS, IHC (fro) 宿主: 小鼠 Monoclonal 5a-8 Biotin
产品编号 ABIN114251
发货至: 中国
  • 抗原 See all CD90 (THY1) 抗体
    CD90 (THY1)
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    小鼠
    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD90 antibody is conjugated to Biotin
    应用范围
    • 170
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    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    This antibody detects CD90 (Thy 1.2). It reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (i.e. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype (i.e. AKR/J, B6.PL (74 NS).
    交叉反应 (详细)
    Species reactivity (tested):Mouse.
    纯化方法
    Protein G Chromatography.
    免疫原
    CBA/J Donor: AKR/J Spleen
    克隆位点
    5a-8
    亚型
    IgG2b
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    Discover our top product THY1 Primary Antibody
  • 应用备注
    Flow Cytometry (protocol see below). Appropriate control samples should always be included in any labelling studies.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    实验流程
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2-0. 5 µg of this antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1/500 dilution. 9. Incubate tubes at 4°C for 30-60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: CBA/JCell Concentration : 1x10e6 cells per testsAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2b,kCell Source: Percentage of cells stained above control: Thymus: 97. 8%Spleen: 35. 4%
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the antibody undiluted (in aliquots) at -20 °C.
  • 抗原
    CD90 (THY1)
    别名
    CD90 (THY1 产品)
    别名
    CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody
    背景
    CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
    基因ID
    21838
    NCBI登录号
    NP_033408
    UniProt
    P01831
    途径
    Cell-Cell Junction Organization
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