CD3 抗体
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北京 101111
Quick Overview for CD3 抗体 (ABIN114132)
抗原
See all CD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Anti-mouse CD3e monoclonal antibody is specific for a 25 kDa protein component (e-T3) of the antigen specific T cell receptor on all mouse strains tested.
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纯化方法
- Purified from ascitic fluid via Protein G Chromatography
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过滤
- 0.2 μm filtered
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免疫原
- H-2Kb sp
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亚型
- IgG
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (APC)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 APC
anti-CD3 (CD3) antibody (PE)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PE
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
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应用备注
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This antibody will prove useful in studying the role of various components of the TCRcomplex in T cell activation and development, and will allow for the development of ananimal model in which to investigate the immunoregulatory effects of in vivoadministration of anti-CD3 antibodies, an area of obvious clinical importance. Anti-CD3 isideal for flow cytometry applications, particularly as a specific marker for tracking mouse Tcells. In addition, this monoclonal antibody, clone 145-2C11 was specifically designed totrigger T cell activation. This clone has also been reported to work in Immunoprecipitation(1, 2) and Western Blotting (Salvadori S. et al.1994. J. of Immunol.153: 5176-5182).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Antibody Concentration: 1 µg/10e6 cellsStrains Tested: BALB/c, C57BL/6, C3H/He, AKR/JPositive control: BALB/c, C57BL/6, C3H/He, AKR/JFlow CytometryAnalysis: 1. Prepare cell suspension in Media A. For cell preparations, deplete the redblood cell population with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration 2x107 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x106 cells, representing one test). 4. To each tube add 0. 2 µg of ABIN114132 per 1x106 cells*. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100µl of secondary antibody (f. e. FITC Goat anti-hamster Ig) at a dilutionrecommended by the manufacturer. PLEASE NOTE: Do not use PE Goat a hamster IgG as the secondary antibody. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in phosphate buffered saline. (This stains dead cells by intercalatingDNA). Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodiumazide ( 100 µl of 2 M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% bovine serum albumin + sodium azide (100 µl
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (0.2 μm filtered), with no preservative
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
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有效期
- 6 months
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- CD3
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别名
- CD3
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背景
- The e-T3 protein has been shown to be non-covalently associated on the cell surface alpha/beta heterodimer of the CD3 associated complex. This monoclonal antibody reacts with all mature T cells and can both activate and inhibit T cell function (1). This fact identifies e-T3 as a cell surface protein involved in the transduction of activation signals. All peripheral T cells express this determinant however B cells and bone marrow cells have proven to be negative. Although the expression of this particular epitope on peripheral T cells is uniformly high, staining of thymocytes reveals distinct subpopulations of cells differing in the level of expression of this marker.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
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基因ID
- 12501
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NCBI登录号
- NP_031674
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UniProt
- P22646
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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