This antibody is specific for Fibronectin (100 %). Collagen type I, III, IV and VI < 0.1 % (RIA at 1/10000 dilution). This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.
Immunofluorescence: 1/80. Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8 °C overnight. ELISA (> / = 1/200). RIA. Positive Control: Human skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 0.5 mL distilled water.
浓度
1.0 mg/mL
缓冲液
Phosphate buffered solution without BSA or preservatives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
Fibronectin
背景
Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1