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Fibronectin 抗体

This anti-Fibronectin antibody is a 兔 多克隆 antibody detecting Fibronectin in IF, IHC (p), EIA, IHC (fro) 和 RIA. Suitable for 人.
产品编号 ABIN113992
发货至: 中国

Quick Overview for Fibronectin 抗体 (ABIN113992)

抗原

See all Fibronectin 抗体
Fibronectin

适用

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宿主

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克隆类型

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多克隆

标记

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This Fibronectin antibody is un-conjugated

应用范围

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Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)
  • 特异性

    This antibody is specific for Fibronectin (100 %). Collagen type I, III, IV and VI < 0.1 % (RIA at 1/10000 dilution). This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

    纯化方法

    Affinity Chromatography.

    免疫原

    Purified fibronectin from human plasma
  • 应用备注

    Immunofluorescence: 1/80. Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8 °C overnight. ELISA (> / = 1/200). RIA. Positive Control: Human skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 0.5 mL distilled water.

    浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered solution without BSA or preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Fibronectin

    背景

    Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

    基因ID

    2335

    NCBI登录号

    NP_002017

    UniProt

    P02751
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