Elastin 抗体
Quick Overview for Elastin 抗体 (ABIN113991)
抗原
See all Elastin (ELN) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody is specific for Human elastin. Cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V < 0.1 %, human fibronectin < 0.1 % . Cross reaction with rat < 0.1 %.
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纯化方法
- Affinity chromatography
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免疫原
- Purified elastin from human skin
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anti-Elastin (ELN) (AA 681-786) antibody
ELN 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (AA 392-645) antibodyELN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) (AA 266-443) antibodyELN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, StM 宿主: 兔 Monoclonal ELN-3131R unconjugated Recombinant Antibody
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal ELN-1981 unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人 IHC, ELISA, IHC (p), IF, RIA 宿主: 兔 Polyclonal unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-648R unconjugated Recombinant Antibody
anti-Elastin (ELN) (AA 392-645) antibodyELN 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Elastin (ELN) antibodyELN 适用: 人, 小鼠, Cow, 小鸡 WB, IHC, IP, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 10B8 unconjugated
anti-Elastin (ELN) (AA 27-200) antibodyELN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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IF: 1: 80. IHC: 1: 500 for paraffin sections, 60 min at RT or 2-8C over night. ELISA: > 1: 200. RIA. Positive control: Human skin or aorta. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Reconstitute with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.
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浓度
- App. 1 mg/mL
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缓冲液
- Phosphate buffered solution, no BSA and preservative added
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Elastin (ELN)
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别名
- Elastin
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背景
- Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties.Synonyms: ELN, Tropoelastin
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基因ID
- 2006
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NCBI登录号
- NP_000492
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UniProt
- P15502
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途径
- Regulation of Actin Filament Polymerization
抗原
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