Fibronectin 抗体
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北京 101111
Quick Overview for Fibronectin 抗体 (ABIN113981)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应 (详细)
- Species reactivity (tested):Mouse.
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纯化方法
- Affinity Chromatography.
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免疫原
- Purified fibronectin from Murine plasma.
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应用备注
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This antibody can be used on Frozen and Paraffin Embedded Tissues: IFA (1/80), IHC(P)(1/500) After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Incubation time: IHC(P) 60 min at RT or 2-8 °C over night. Also suitable for ELISA (1/200) and RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water, dilute further with appropriate antibody diluent.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS without preservative or stabilizer.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Fibronectin
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背景
- Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1
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基因ID
- 10090
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UniProt
- P11276
抗原
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