Immunohistochemistry on Paraffin Sections: 1/200-1/600. Immunofluorescence: 1/80. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Rat Skin, Liver. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Also suitable for use in RIA and ELISA: >/= 1/200 (OD >/=500). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 0.5 mL distilled water.
浓度
1.0 mg/mL
缓冲液
PBS without BSA or preservatives.
储存液
Without preservative
储存条件
4 °C
储存方法
Store at 2-8 °C.
抗原
Fibronectin
背景
Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumors.Synonyms: CIG, Cold-insoluble globulin, FN1