Fibronectin 抗体
Quick Overview for Fibronectin 抗体 (ABIN113980)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography.
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免疫原
- Purified Fibronectin from Rat plasma.
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应用备注
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Immunohistochemistry on Paraffin Sections: 1/200-1/600. Immunofluorescence: 1/80. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Rat Skin, Liver. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Also suitable for use in RIA and ELISA: >/= 1/200 (OD >/=500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS without BSA or preservatives.
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储存液
- Without preservative
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C.
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- Fibronectin
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背景
- Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumors.Synonyms: CIG, Cold-insoluble globulin, FN1
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基因ID
- 10116
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UniProt
- P04937
抗原
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