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Fibronectin 抗体

This anti-Fibronectin antibody is a 兔 多克隆 antibody detecting Fibronectin in IHC (p), EIA, IHC (fro) 和 RIA. Suitable for 大鼠.
产品编号 ABIN113980
发货至: 中国

Quick Overview for Fibronectin 抗体 (ABIN113980)

抗原

See all Fibronectin 抗体
Fibronectin

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This Fibronectin antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)
  • 纯化方法

    Affinity Chromatography.

    免疫原

    Purified Fibronectin from Rat plasma.
  • 应用备注

    Immunohistochemistry on Paraffin Sections: 1/200-1/600. Immunofluorescence: 1/80. Incubation Time: 60 min at RT or 2-8 °C over night. Positive Control: Rat Skin, Liver. Pretreatment: After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blockingserum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBSfor 30 min at RT is recommended. Also suitable for use in RIA and ELISA: >/= 1/200 (OD >/=500).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 0.5 mL distilled water.

    浓度

    1.0 mg/mL

    缓冲液

    PBS without BSA or preservatives.

    储存液

    Without preservative

    储存条件

    4 °C

    储存方法

    Store at 2-8 °C.
  • 抗原

    Fibronectin

    背景

    Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumors.Synonyms: CIG, Cold-insoluble globulin, FN1

    基因ID

    10116

    UniProt

    P04937
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