IF: 1: 40. IHC: 1: 200 - 1: 600 for paraffin sections, 60 min at RT or 2-8C over night. ELISA. RIA. Positive control: Chicken skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Reconstitute with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.
浓度
App. 1 mg/mL
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件
4 °C/-20 °C
储存方法
Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
Fibronectin
背景
Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1