Fibronectin 抗体
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北京 101111
Quick Overview for Fibronectin 抗体 (ABIN113978)
抗原
See all Fibronectin 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Purified
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免疫原
- Purified fibronectin from bovine plasma
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应用备注
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IF: 1: 40. IHC: 1: 200 - 1: 600 for paraffin sections, 60 min at RT or 2-8C over night. ELISA. RIA. Positive control: Bovine skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Reconstitute with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.
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浓度
- App. 1 mg/mL
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Fibronectin
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背景
- Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1
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基因ID
- 9913
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UniProt
- P07589
抗原
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