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Fibronectin 抗体

This anti-Fibronectin antibody is a 兔 多克隆 antibody detecting Fibronectin in IHC (p), EIA, IHC (fro) 和 RIA. Suitable for Cow.
产品编号 ABIN113978
发货至: 中国

Quick Overview for Fibronectin 抗体 (ABIN113978)

抗原

See all Fibronectin 抗体
Fibronectin

适用

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Cow

宿主

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克隆类型

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多克隆

标记

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This Fibronectin antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)
  • 纯化方法

    Purified

    免疫原

    Purified fibronectin from bovine plasma
  • 应用备注

    IF: 1: 40. IHC: 1: 200 - 1: 600 for paraffin sections, 60 min at RT or 2-8C over night. ELISA. RIA. Positive control: Bovine skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Reconstitute with 0.5 mL distilled water, for further dilution use appropriate antibody diluent.

    浓度

    App. 1 mg/mL

    注意事项

    Avoid repeated freezing and thawing. Dilute only prior to immediate use

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Fibronectin

    背景

    Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.Synonyms: CIG, Cold-insoluble globulin, FN1

    基因ID

    9913

    UniProt

    P07589
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