Collagen Type V 抗体
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Quick Overview for Collagen Type V 抗体 (ABIN113975)
抗原
See all Collagen Type V (COL5) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Rat Collagen type V: 100 % Rat Collagen type I, II and II: < 0.1 % Rat Elastin: < 0.1 % (RIA at 1/100 dilution).
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纯化方法
- Ion Exchange Chromatography.
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免疫原
- Purified collagen type V from rat skin.
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anti-Collagen, Type V (COL5) antibody
COL5 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) (AA 1450-1500) antibodyCOL5 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibodyCOL5 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type V (COL5) (AA 61-90), (N-Term) antibodyCOL5 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人, Cow WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
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应用备注
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RIA. ELISA: > 1/200 (OD > 500). Immunofluorescence: 1/40. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissues: 1/500. Incubation time: 60 min. at RT or 2-8 °C overnight. Positive Control: Rat skin or liver. Pretreatment: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase(app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked byblocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxidesolution in TBS for 30 min at RT is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water. For further dilution use appropriate antibody diluent.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS without BSA and preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- Collagen Type V (COL5) (Collagen, Type V (COL5))
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别名
- Collagen Type V
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背景
- Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III.Synonyms: COL5A1
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基因ID
- 85490
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NCBI登录号
- NP_604447
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UniProt
- Q9JI03
抗原
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