NPY1R 抗体 (AA 365-378)
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Quick Overview for NPY1R 抗体 (AA 365-378) (ABIN113620)
抗原
See all NPY1R 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 365-378
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特异性
- This antibody reacts with Neuropeptide Y Receptor 1.
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纯化方法
- Ammonium Sulphate Fractionation
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免疫原
- Synthetic peptide corresponding to amino acids 365-378 of human NPY1R (KQASPVAFKKINNN) conjugated to KLH.
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应用备注
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ELISA. Western blot (1/250 as a guide). Immunohistochemistry on Paraffin Sections (1/1,000-1/4,000): See below for protocol.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol: - Samples: Bouin's fixed paraffin sections, 5 µm, from rat brain. - 5 minutes microwave pre-treatment, citrate buffer, pH 6. 0. - Peroxidase blocking (15 minutes), normal rabbit serum (30 minutes),
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 5.0 mg/mL
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缓冲液
- PBS, pH 7.2, containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- Do Not Add Sodium Azide
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
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- NPY1R (Neuropeptide Y Receptor 1 (NPY1R))
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别名
- Neuropeptide Y Receptor 1
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背景
- Neuropeptide Y (NPY) is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system. There are two major subtypes of NPY (Y1 and Y2). The NPY Y1 receptors have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, breast, colon, ganglion, heart, and aortic smooth muscle. The Y2 receptor is found mainly in the central nervous system. NPY receptors are a member of the G protein-coupled receptor superfamily.Synonyms: NPYR, NPYY1, Neuropeptide Y receptor 1
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基因ID
- 4886
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NCBI登录号
- NP_000900
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UniProt
- P25929
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途径
- Feeding Behaviour
抗原
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