The antiserum stains specifically Human hair keratin K33 expressed starting from the middle hair cortex. Because of high sequence homology both Keratin isoforms K33a (formerly also designated keratin hHa3-I) and K33b (formerly designated keratin hHa3-II) are detected.
纯化方法
Liquid serum
免疫原
Synthetic peptide of human hair (trichocytic) keratin hHa3 (EKP IGS CVT NPC GPR SR), coupled to KLH.
Immunohistochemistry on frozen sections: 1/200, 1h at RT, preincubate fixed sections with0.1 Triton X-100 (in PBS) for 1 - 5 min prior to first antibody incubation step. Immunoblotting: 1 : 2,000, ECL method. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
Keratin 33A (KRT33A)
别名
Keratin-33A (KRT33A)
背景
Synonyms: HHA3-I, HKA3A, Hair keratin type I Ha3-I, K33A, KRTHA3A, Keratin type I cuticular Ha3-I