Affinity purified against peptide coupled to Sepharose
免疫原
Synthetic C-terminal peptide coupled to carrier protein Remarks: The immunizing sequence is > 90% identical with the corresponding mouse and rat sequences.
Immunohistochemistry on frozen sections: 1 μg/mL (1: 200). Lower working concentrationcombined with longer incubation time (e. g. > 2 hours at room temperature) and choice ofblocking reagent and secondary antibody may improve your results. Recommended positive control: Rat brain Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 0.5 mL distilled water to make stock solution.
浓度
0.2 mg/mL
缓冲液
PBS, pH 7.2 containing 0.01 % Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Fractalkine is a chemokine present in endothelial cells from several tissues, including brain, liver, and kidney. A high level of fractalkine has also been described in neurons from different regions of the mouse brain. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membranespanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR(1).Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1