Lipopolysaccharides (LPS) 抗体
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Quick Overview for Lipopolysaccharides (LPS) 抗体 (ABIN113127)
抗原
See all Lipopolysaccharides (LPS) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Sodium Sulphate Precipitation and Ion Exchange Chromatography
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免疫原
- Whole cells prep of Lipid A from E. coli O157
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应用备注
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Indirect Immunofluoresence: > 1/100 (against members of the Entero-bacteriaciae). May also be used in place of neat antiserum in almost any appropriate antibody-basedtechnique. Also suitable for conjugation purposes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 4-5 mg/mL (OD280 nm, E0.1% = 1.4)
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缓冲液
- 0.01 M PBS, pH 7.2, 0.09 % Sodium Azide, None
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Lipopolysaccharides (LPS)
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物质类
- Chemical
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背景
- Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.Synonyms: LPS, Lipid A
抗原
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