Lactoferrin 抗体 (Biotin)
Quick Overview for Lactoferrin 抗体 (Biotin) (ABIN113033)
抗原
See all Lactoferrin (LTF) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Immunoaffinity chromatography using antigens coupled to agarose beads.
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应用备注
- A dilution range of 1: 20,000 - 1: 400,000 is suggested for ELISA and Western blotting usingenzyme-conjugated streptavidin,1: 500 - 1: 5,000 for enzyme immunohistochemistry on tissue sections and 1: 200 - 1: 1,000for flow cytometry and fluorescence immunohisto/cytochemistry.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Reconstitute to 1.5 mL with distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temp. To judge clarity by drawing product into a Pasteur pipette.
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浓度
- 1.2 mg/mL (prior to lyophilization)
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缓冲液
- 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 containing 0.01 % Thimerosal, 0.05 % Sodium Azide as preservative and 15 mg/mL BSA as stabilizer.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2 - 8 °C.
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- Lactoferrin (LTF) (Lactotransferrin (LTF))
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别名
- Lactotransferrin
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背景
- Lactoferrin belongs to a family of iron-binding proteins that modulate iron metabolism, hemopoiesis, and immunologic reactions, together with transferrin and melanoma tumor antigen p97. They are evolutionary products of gene duplication and all 3 are encoded by genes on 3q. Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.Synonyms: EC=3.4.21, LF, LTF, Lactoferrin, Talalactoferrin
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基因ID
- 9606
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UniProt
- P02788
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途径
- Transition Metal Ion Homeostasis
抗原
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