Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
特异性
Specific for the C-term of the M3 receptor (580-589): 100 % . Crossreactivity to full receptor 70 %, M1 (451-460), M2 (457-466), M4 (469-478) and M5 (519-531) < 0.01 %.
免疫原
Synthetic peptide M3 (580-589) covalently attached to a carrier protein.
Immunocytochemical: this product has been found to stain PLP fixed rat brain sectionsknown to express the muscarinic M3 receptor using ABC techniques at a dilution of1: 2,500. Western immunoblotting: successful by using whole rat brain homogenerate at a dilutionof 1: 500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
溶解方式
Restore with 0.1 mL PBS containing 10 mg/mL BSA or with additional buffer for more dilute antibody.
储存液
Azide free
注意事项
Do Not Use Sodium Azide as preservative.
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件
4 °C/-20 °C
储存方法
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
This receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3