CHRM3 抗体 (AA 580-589)
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北京 101111
Quick Overview for CHRM3 抗体 (AA 580-589) (ABIN112905)
抗原
See all CHRM3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 580-589
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特异性
- Specific for the C-term of the M3 receptor (580-589): 100 % . Crossreactivity to full receptor 70 %, M1 (451-460), M2 (457-466), M4 (469-478) and M5 (519-531) < 0.01 %.
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免疫原
- Synthetic peptide M3 (580-589) covalently attached to a carrier protein.
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应用备注
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Immunocytochemical: this product has been found to stain PLP fixed rat brain sectionsknown to express the muscarinic M3 receptor using ABC techniques at a dilution of1: 2,500. Western immunoblotting: successful by using whole rat brain homogenerate at a dilutionof 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 0.1 mL PBS containing 10 mg/mL BSA or with additional buffer for more dilute antibody.
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储存液
- Azide free
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注意事项
- Do Not Use Sodium Azide as preservative.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))
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别名
- Muscarinic Acetylcholine Receptor M3
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背景
- This receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Expression of this receptor has been reported in brain, eye, and heart. ESTs have been isolated from brain, colon, fetus, lung, and prostate libraries.Synonyms: CHRM3
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基因ID
- 1131
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NCBI登录号
- NP_000731
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UniProt
- P20309
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途径
- Synaptic Membrane
抗原
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