Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: Strong reaction at 1/20 dilution, No antigenpreservation required, 35 minutes recommended incubation time. ELISA. Western Blot: In blots, a major band is obtained at 50 kD with minor bands at 35 kD, 40 kdand 67 kD, This reflects bands found in DTT reduced Blots of the immunising antigen andin immunoblots using a known antibody positive serum. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.