Mitotic Cells 抗体
适用: 人, 斑马鱼
FACS, IHC (fro), IF
宿主: 小鼠
Monoclonal
8B3G
unconjugated
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- 抗原
- Mitotic Cells
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适用
- 人, 斑马鱼
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- 交叉反应 （详细）
- Species reactivity (tested):Human, Zebrafish.
- 纯化方法
- Immunoaffinity Chromatography
- 免疫原
- Total cell lysate of the human bladder carcinoma cell line T24.
- 克隆位点
- 8B3G
- 亚型
- IgM
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anti-Mitotic Cells antibody
适用: 人, 斑马鱼 FACS, IHC, ICC 宿主: 小鼠 Monoclonal 8B3G unconjugated
anti-Mitotic Cells antibody适用: 人 FACS, IHC, ICC 宿主: 小鼠 Monoclonal 8B3G unconjugated
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- 应用备注
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This antibody is suitable for Flow cytometric analyses (1/50-1/100) andImmunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent (1/50-1/100). Not suitable for Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the lyophilized antibody at 2-8 °C for up to one month or at -20 °C for longer.
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- 抗原
- Mitotic Cells
- 背景
- The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a 'quiescent' phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase. M phase itself is highly regulated, and is divided into five stages: prophase, prometaphase, metaphase, telophase and anaphase.
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