Mitotic Cells 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS, IHC (fro) 和 IF. Suitable for 人 和 斑马鱼.
Quick Overview for Mitotic Cells 抗体 (ABIN112395)
抗原
Mitotic Cells
适用
人, 斑马鱼
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
克隆位点
8B3G
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交叉反应 （详细）
- Species reactivity (tested):Human, Zebrafish.
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- Total cell lysate of the human bladder carcinoma cell line T24.
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亚型
- IgM
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anti-Mitotic Cells antibody
适用: 人, 斑马鱼 FACS, ICC, IHC 宿主: 小鼠 Monoclonal 8B3G unconjugated
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应用备注
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This antibody is suitable for Flow cytometric analyses (1/50-1/100) andImmunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent (1/50-1/100). Not suitable for Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the lyophilized antibody at 2-8 °C for up to one month or at -20 °C for longer.
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- Mitotic Cells
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背景
- The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a 'quiescent' phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase. M phase itself is highly regulated, and is divided into five stages: prophase, prometaphase, metaphase, telophase and anaphase.
抗原
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