Reticulon 1 抗体 (N-Term)
Quick Overview for Reticulon 1 抗体 (N-Term) (ABIN112389)
抗原
See all Reticulon 1 (RTN1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- RNL-4 recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C). RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.
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纯化方法
- Purified
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免疫原
- Synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
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亚型
- IgG1
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anti-Reticulon 1 (RTN1) (AA 421-589) antibody
RTN1 适用: 人, 兔, 猴 WB, IHC, IHC (fro), IHC (p), ICC, FACS 宿主: 小鼠 Monoclonal RNL-2 unconjugated
anti-Reticulon 1 (RTN1) (C-Term) antibodyRTN1 适用: 人, 小鼠, 大鼠, 兔, 猴, 犬, 豚鼠, 斑马鱼, Pig, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 36-85) antibodyRTN1 适用: 人, 小鼠, 大鼠, 猴, Cow, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 80-340) antibodyRTN1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (N-Term) antibodyRTN1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 589-776) antibodyRTN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 593-780) antibodyRTN1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 198-227), (N-Term) antibodyRTN1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB36663 unconjugated
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应用备注
- RNL-4 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and Flow cytometry. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted -20 °C to -70 °C.
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- Reticulon 1 (RTN1)
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别名
- Reticulon 1 / RTN1
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背景
- Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1
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基因ID
- 6252
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NCBI登录号
- NP_066959
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UniProt
- Q16799
抗原
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