Reticulon 1 抗体
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Quick Overview for Reticulon 1 抗体 (ABIN112386)
抗原
See all Reticulon 1 (RTN1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- RNL-2 recognizes an epitope located within the region of amino acids 421-589 of the neuroendocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, RNL-2 reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining.
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交叉反应 （详细）
- Species reactivity (tested):Human, Rabbit and Rhesus Monkey.
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纯化方法
- Purified
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免疫原
- The small cell lung cancer cell line NCI-H82.
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亚型
- IgG1
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anti-Reticulon 1 (RTN1) (AA 421-589) antibody
RTN1 适用: 人, 兔, 猴 WB, IHC, IHC (fro), ICC, IHC (p), FACS 宿主: 小鼠 Monoclonal RNL-2 unconjugated
anti-Reticulon 1 (RTN1) (C-Term) antibodyRTN1 适用: 人, 小鼠, 大鼠, 兔, 猴, 犬, 豚鼠, 斑马鱼, Pig, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 36-85) antibodyRTN1 适用: 人, 小鼠, 大鼠, 猴, Cow, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (N-Term) antibodyRTN1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 589-776) antibodyRTN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 593-780) antibodyRTN1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 198-227), (N-Term) antibodyRTN1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB36663 unconjugated
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应用备注
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Suitable for Immunocytochemistry, Immunohistochemistry on frozen tissues,Immunoblotting and Flow cytometry. Recommended dilutions: 1/25-1/200 for Flow cytometry, and for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted -20 °C only.
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- Reticulon 1 (RTN1)
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别名
- Reticulon 1 / RTN1
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背景
- Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1
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基因ID
- 6252
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NCBI登录号
- NP_066959
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UniProt
- Q16799
抗原
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