Reticulon 1 抗体
Our Local Distributor
北京 101111
Quick Overview for Reticulon 1 抗体 (ABIN112385)
抗原
See all Reticulon 1 (RTN1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- MON-162 exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues.
-
纯化方法
- Purified
-
免疫原
- Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
-
亚型
- IgG1
-
-
anti-Reticulon 1 (RTN1) (AA 198-227), (N-Term) antibody
RTN1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB36663 unconjugated
anti-Reticulon 1 (RTN1) (AA 421-589) antibodyRTN1 适用: 人, 兔, 猴 WB, IHC, IHC (fro), ICC, IHC (p), FACS 宿主: 小鼠 Monoclonal RNL-2 unconjugated
anti-Reticulon 1 (RTN1) (C-Term) antibodyRTN1 适用: 人, 小鼠, 大鼠, 兔, 猴, 犬, 豚鼠, 斑马鱼, Pig, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 36-85) antibodyRTN1 适用: 人, 小鼠, 大鼠, 猴, Cow, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (N-Term) antibodyRTN1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 593-780) antibodyRTN1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 1 (RTN1) (AA 589-776) antibodyRTN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Suitable for Immunocytochemistry, Immunohistochemistry on frozen and paraffinembedded tissue, and Immunoblotting. Recommended dilutions: 1/100-1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent and 1/100-1/1000 for Immunoblotting applications.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- PBS with 0.09 % sodium azide as preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing. Do not freeze working dilutions.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store lyophilized antibody at 2-8 °C. Aliquot and store reconstituted antibody at -20 °C.
-
-
- Reticulon 1 (RTN1)
-
别名
- Reticulon 1 / RTN1
-
背景
- Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSPA), Reticulon-1B (NSP-B), and Reticulon-1C (NSPC). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non- SCLC (cell cultures). The epitope of MON-162 is located between amino acid residues 338-422 of Reticulon-1A.Synonyms: NSP, Neuroendocrine-specific protein, RTN1, Reticulon-1
-
基因ID
- 6252
-
NCBI登录号
- NP_066959
-
UniProt
- Q16799
抗原
-
