Plakophilin 3 抗体 (N-Term)
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Quick Overview for Plakophilin 3 抗体 (N-Term) (ABIN112378)
抗原
See all Plakophilin 3 (PKP3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 570-589, N-Term
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特异性
- This antibody 23E3/4 reacts with an epitope located between residues 570-589 in Human Plakophilin-3.
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse and Canine (Dog).
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纯化方法
- Purified
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免疫原
- Synthetic peptide corresponding to amino acid residues 570-589 of Human Plakophilin-3 coupled to Keyhole Limpet Hemocyanin via an additional cysteine residue at the N- terminus (CFTPQSRRLRELPLAADALTF).
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亚型
- IgG2b
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anti-Plakophilin 3 (PKP3) (AA 225-324) antibody
PKP3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6E5 unconjugated
anti-Plakophilin 3 (PKP3) antibodyPKP3 适用: 人, 小鼠, 犬 WB, IHC, IP, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal 23E3-4 unconjugated
anti-Plakophilin 3 (PKP3) (C-Term) antibodyPKP3 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Plakophilin 3 (PKP3) (AA 225-324) antibodyPKP3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Plakophilin 3 (PKP3) (AA 570-589) antibodyPKP3 适用: 人, 小鼠, 犬 WB, IHC, IP, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal 23E3-4 unconjugated
anti-Plakophilin 3 (PKP3) (AA 1-797) antibodyPKP3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Plakophilin 3 (PKP3) (AA 1-308) antibodyPKP3 适用: 人, 小鼠 WB, IP, IHC (fro), HCP 宿主: 小鼠 Monoclonal 7F6 unconjugated
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应用备注
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This antibody is suitable for Immunocytochemistry (methanol-and paraformaldehyde-fixedcells), Immunohistochemistry on frozen and paraffin embedded tissues,Immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2 % Triton-X 100for, respectively, 5 min and 15 min prior to incubation with primary antibody. Recommended dilutions: Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. Immunoblotting: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS with 0.09 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- Plakophilin 3 (PKP3)
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别名
- Plakophilin 3 / PKP3
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背景
- Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin- 3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin- 3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.Synonyms: Plakophilin-3
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基因ID
- 11187
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NCBI登录号
- NP_009114
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UniProt
- Q9Y446
抗原
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