Plakophilin 3 抗体 (N-Term)
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- 抗原 See all Plakophilin 3 (PKP3) 抗体
- Plakophilin 3 (PKP3)
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抗原表位
- AA 779-793, N-Term
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适用
- 人, 小鼠, 犬, Pig
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Plakophilin 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- 特异性
- This antibody reacts with an epitope located between residues 779-793 in Human Plakophilin-3.
- 纯化方法
- Purified
- 免疫原
- Synthetic peptide corresponding to amino acid residues 779-793 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CKLHRDFAKGYRKED).
- 克隆位点
- 12B11F8
- 亚型
- IgG2a
- Top Product
- Discover our top product PKP3 Primary Antibody
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- 应用备注
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E612B11F8 is suitable for Immunocytochemistry (methanol-fixed cells),Immunohistochemistry on frozen tissues, Immunoprecipitation and Western blotdetection. For optimal results, fixed cells or tissues should be treated with 0.2 % Triton-X 100 for 5 min. prior to incubation with primary antibody. Recommended dilutions: 1/25-1/200 for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 forImmunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS with 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store lyophilized at 2-8 °C and reconstituted at -20 °C.
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- 抗原
- Plakophilin 3 (PKP3)
- 别名
- Plakophilin 3 / PKP3 (PKP3 产品)
- 背景
- Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.Synonyms: Plakophilin-3
- 基因ID
- 11187
- NCBI登录号
- NP_009114
- UniProt
- Q9Y446
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