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Plakophilin 3 抗体 (N-Term)

This anti-Plakophilin 3 antibody is a 小鼠 单克隆 antibody detecting Plakophilin 3 in WB, IP, IHC (fro) 和 IF. Suitable for 人, 小鼠, 犬 和 Pig.
产品编号 ABIN112377
发货至: 中国

Quick Overview for Plakophilin 3 抗体 (N-Term) (ABIN112377)

抗原

See all Plakophilin 3 (PKP3) 抗体
Plakophilin 3 (PKP3)

适用

  • 31
  • 8
  • 5
  • 2
  • 1
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人, 小鼠, 犬, Pig

宿主

  • 16
  • 13
  • 2
小鼠

克隆类型

  • 17
  • 14
单克隆

标记

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  • 1
  • 1
  • 1
  • 1
  • 1
This Plakophilin 3 antibody is un-conjugated

应用范围

  • 31
  • 13
  • 12
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  • 9
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  • 5
  • 5
  • 2
  • 1
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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

克隆位点

12B11F8
  • 抗原表位

    • 9
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 779-793, N-Term

    特异性

    This antibody reacts with an epitope located between residues 779-793 in Human Plakophilin-3.

    纯化方法

    Purified

    免疫原

    Synthetic peptide corresponding to amino acid residues 779-793 of human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CKLHRDFAKGYRKED).

    亚型

    IgG2a
  • 应用备注

    E612B11F8 is suitable for Immunocytochemistry (methanol-fixed cells),Immunohistochemistry on frozen tissues, Immunoprecipitation and Western blotdetection. For optimal results, fixed cells or tissues should be treated with 0.2 % Triton-X 100 for 5 min. prior to incubation with primary antibody. Recommended dilutions: 1/25-1/200 for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 forImmunoblotting applications.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS with 0.09 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store lyophilized at 2-8 °C and reconstituted at -20 °C.
  • 抗原

    Plakophilin 3 (PKP3)

    别名

    Plakophilin 3 / PKP3

    背景

    Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells [1]. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of 87 kD.Synonyms: Plakophilin-3

    基因ID

    11187

    NCBI登录号

    NP_009114

    UniProt

    Q9Y446
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