ITGB1 抗体 (C-Term, N-Term)
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Quick Overview for ITGB1 抗体 (C-Term, N-Term) (ABIN112375)
抗原
See all ITGB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term, N-Term
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纯化方法
- Purified
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免疫原
- 1G2 is a mouse monoclonal IgG2a, k antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin b1D including an appending N-terminal cysteine (CQENPIYKS-PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.
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亚型
- IgG2a
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应用备注
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Suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry on frozensections. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS with 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store lyophilized antibody at -20 °C to -70 °C. Reconstituted antibody should be stored in working aliquots at -20 °C.
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- ITGB1 (Integrin beta 1 (ITGB1))
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别名
- CD29 / Integrin beta-1
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背景
- Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated a and b subunits. More than 18 a and 8 b subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. There are two major forms of integrin b1: b1A and b1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. b1A is present in all tissues, except cardiac and skeletal muscle, which instead express the b1D variant.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12
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基因ID
- 9606
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UniProt
- P05556
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途径
- Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome
抗原
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