NEFM 抗体 (phosphospecific)
Quick Overview for NEFM 抗体 (phosphospecific) (ABIN112358)
抗原
See all NEFM 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- phosphospecific
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特异性
- RNF406 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein. Reacts with Human, Rabbit, Hamster, Monkey, Guinea Pig and Xenopus.
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纯化方法
- Purified
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免疫原
- Neurofilament preparation of calf brain tissue.
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亚型
- IgG1
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anti-Neurofilament Triplet M Protein (NEFM) (AA 779-916) antibody
NEFM 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1B6B12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyNEFM 适用: 哺乳动物 WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal NF-09 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 381-443) antibodyNEFM 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 101-200) antibodyNEFM 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 105-287) antibodyNEFM 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (AA 200-300) antibodyNEFM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (C-Term) antibodyNEFM 适用: 大鼠 WB, IF 宿主: 小鸡 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (C-Term) antibodyNEFM 适用: 大鼠 WB, IF 宿主: 小鼠 Monoclonal 3H11 unconjugated
anti-Neurofilament Triplet M Protein (NEFM) (Internal Region) antibodyVerified NEFM 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Neurofilament Triplet M Protein (NEFM) antibodyNEFM 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
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应用备注
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RNF406 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen sections and paraffin-embedded tissues and Flow cytometry. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS with 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store lyophilized at 2 - 8 °C and reconstituted at -20 °C.
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- NEFM (Neurofilament Triplet M Protein (NEFM))
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别名
- Neurofilament M (160 kD)
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背景
- Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide
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基因ID
- 4741
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NCBI登录号
- NP_005373
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UniProt
- P07197
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途径
- Brown Fat Cell Differentiation
抗原
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