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NEFM 抗体

NEFM 适用: 人, 大鼠, 猴, Hamster, 非洲爪蟾 WB, IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal RNF403 unconjugated
产品编号 ABIN112357
发货至: 中国
  • 抗原 See all NEFM 抗体
    NEFM (Neurofilament Triplet M Protein (NEFM))
    适用
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    人, 大鼠, 猴, Hamster, 非洲爪蟾
    宿主
    • 40
    • 24
    • 12
    • 1
    小鼠
    克隆类型
    • 44
    • 33
    单克隆
    标记
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    This NEFM antibody is un-conjugated
    应用范围
    • 69
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    RNF403 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.
    纯化方法
    Purified
    免疫原
    Neurofilament preparation of calf brain tissue.
    克隆位点
    RNF403
    亚型
    IgG1
    Top Product
    Discover our top product NEFM Primary Antibody
  • 应用备注
    Immunoblotting. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissues. Recommended Dilutions: 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent.1/100-1/500 for Immunoblotting applications.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, 0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    NEFM (Neurofilament Triplet M Protein (NEFM))
    别名
    Neurofilament M (160 kD) (NEFM 产品)
    别名
    NEF3 antibody, NF-M antibody, NFM antibody, Nef3 antibody, Nfm antibody, NF160 antibody, NF165 antibody, nef-m antibody, nef3 antibody, nefm antibody, nf-m antibody, zgc:112359 antibody, nefm-a antibody, nefm-b antibody, neurofilament medium antibody, neurofilament triplet M protein antibody, neurofilament, medium polypeptide antibody, neurofilament medium polypeptide antibody, neurofilament medium L homeolog antibody, neurofilament, medium polypeptide a antibody, neurofilament medium S homeolog antibody, NEFM antibody, Nefm antibody, TVAG_582010 antibody, TVAG_379630 antibody, nefm.L antibody, nefma antibody, nefm.S antibody
    背景
    Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.Synonyms: (Neuronal Marker), NEF3, NEFM, NF-M, Neurofilament 3, Neurofilament medium polypeptide
    基因ID
    4741
    NCBI登录号
    NP_005373
    UniProt
    P07197
    途径
    Brown Fat Cell Differentiation
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