KRT8 抗体
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北京 101111
Quick Overview for KRT8 抗体 (ABIN112350)
抗原
See all KRT8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This Monoclonal antibody H1 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells.
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交叉反应 (详细)
- Species reactivity (tested):Human, Zebrafish.
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纯化方法
- Purified
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免疫原
- Cytoskeleton preparation containing Cytokeratin 8.
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亚型
- IgG1
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应用备注
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Suitable for Immunoblotting, Immunocytochemistry, immunohistochemistry on frozen andparaffin-embedded tissues and flow cytometry. Recommended dilutions: 1/25-1/200 for flow cytometry, and for immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Do not freeze working dilutions.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the original vial at 2-8 °C. After reconstitution store (in aliquots) at -20 °C.
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- KRT8 (Keratin 8 (KRT8))
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别名
- Cytokeratin 8
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背景
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
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基因ID
- 3856
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NCBI登录号
- NP_002264
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UniProt
- P05787
抗原
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