Cytokeratin 13 抗体
Quick Overview for Cytokeratin 13 抗体 (ABIN112324)
抗原
See all Cytokeratin 13 (KRT13) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
 - Ks 13.1 represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Polypeptide reacting: Mr 54 000 polypeptide human keratin K13 (formerly designated cytokeratin13, with minor affinity to keratins K14, Mr 50 000, and K16, Mr 48 000). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma, transitional cell carcinoma of the bladder, craniopharyngioma.
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交叉反应 (详细)
 - Species reactivity (tested):Human, Bovine and Rat.
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纯化方法
 - Protein A affinity chromatography
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免疫原
 - Keratin K13 of Mr 54.000 purified from human esophagus.
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亚型
 - IgG1
 
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应用备注
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                        Immunofluorescence. Immunohistochemistry on frozen and paraffin sections: 1: 10, protease pretreatmentimproves the performance of this excellent marker in immunohisto-chemistry: 1/10 withPBS, pH 7.4. Western Blot. Incubation time: 1h at RT, extended with parafin. 
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 
                                            
限制
 - 仅限研究用
 
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溶解方式
 - Restore in 1 mL dist. water.
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缓冲液
 - PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
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储存液
 - Sodium azide
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注意事项
 - This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Avoid repeated freezing and thawing.
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储存条件
 - 4 °C/-20 °C
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储存方法
 - Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
 
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- Cytokeratin 13 (KRT13) (Keratin 13 (KRT13))
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别名
 - Cytokeratin 13
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背景
 - Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.Synonyms: CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13
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基因ID
 - 3860
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NCBI登录号
 - NP_002265
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UniProt
 - P13646
 
抗原
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