KRT4 抗体
(1 reference)Quick Overview for KRT4 抗体 (ABIN112320)
抗原
See all KRT4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This Monoclonal antibody 6B10 reacts exlcusively with Cytokeratin 4 which is present in non-cornifying squamous epithelium, including cornea and transitional epithelium. Cells in certain ciliated pseudo-stratified epithelia and ductal epithelia of various exocrine glands are also positive for 6B10.
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交叉反应 （详细）
- Species reactivity (tested):Human, Canine, Feline, Zebrafish.
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纯化方法
- Purified
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免疫原
- 6B10 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytokeratin preparation extracted from human esophagus.
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亚型
- IgG1
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anti-Keratin 4 (KRT4) (AA 152-457) antibody
KRT4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 4 (KRT4) (AA 194-300) antibodyKRT4 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5H5 unconjugated
anti-Keratin 4 (KRT4) antibodyKRT4 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 2F9 unconjugated
anti-Keratin 4 (KRT4) (AA 181-292) antibodyKRT4 适用: 人 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal KRT4-2804 unconjugated
anti-Keratin 4 (KRT4) (AA 1-534) antibodyKRT4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 4 (KRT4) (AA 317-454) antibodyKRT4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 4 (KRT4) (AA 256-360) antibodyKRT4 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 4 (KRT4) (AA 1-200) antibodyKRT4 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 4 (KRT4) (AA 386-415), (C-Term) antibodyKRT4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18906 unconjugated
anti-Keratin 4 (KRT4) antibodyKRT4 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunocytochemistry. Immunohistochemistry on Frozen and Tissues (1/25-1/200) using with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent. Immunoblotting (1/100-1/1000). Flow Cytometry (1/25-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "The eyelid margin: a transitional zone for 2 epithelial phenotypes." in: Archives of ophthalmology (Chicago, Ill. : 1960), Vol. 125, Issue 4, pp. 523-32, (2007) (PubMed).
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: "The eyelid margin: a transitional zone for 2 epithelial phenotypes." in: Archives of ophthalmology (Chicago, Ill. : 1960), Vol. 125, Issue 4, pp. 523-32, (2007) (PubMed).
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- KRT4 (Keratin 4 (KRT4))
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别名
- Cytokeratin 4
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背景
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK4, CYK4, Cytokeratin-4, K4, KRT4, Keratin, Keratin-4, type II cytoskeletal 4
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基因ID
- 9606
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UniProt
- P19013
抗原
-
