Uroplakin 3A 抗体
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北京 101111
Quick Overview for Uroplakin 3A 抗体 (ABIN112250)
抗原
See all Uroplakin 3A (UPK3A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Clone AU1 reacts specifically with Uroplakin III present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. About 60 % of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of Uroplakin III. Until now, no Uroplakin staining was found in any non-urothelial carcinoma (Moll et al., 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor.
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交叉反应 （详细）
- Species reactivity (tested):Human, Bovine, Pig and Rat.
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纯化方法
- Affinity Chromatography on Protein A.
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免疫原
- Asymmetric unit membrane (AUM) preparation from bovine urinary bladder.
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亚型
- IgG1
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anti-Uroplakin 3A (UPK3A) (AA 15-212) antibody
UPK3A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 3A (UPK3A) (AA 15-211) antibodyUPK3A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 3A (UPK3A) (AA 19-207) antibodyUPK3A 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 3A (UPK3A) antibodyUPK3A 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 3A (UPK3A) (AA 19-207) antibodyUPK3A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 3A (UPK3A) (AA 260-279) antibodyUPK3A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoblotting (Western blotting). Immunohistochemistry on Paraffin-Embedded Tissue (not on Frozen Sections! ) using aheat-induced retrieval method (e. g. microwave treatment): Working dilution: 1/10. Incubation Time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL distilled water.
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缓冲液
- Final solution contains PBS, pH 7.4 with 0.5 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- Uroplakin 3A (UPK3A)
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别名
- Uroplakin-3a (UPK3A)
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背景
- Together with the Uroplakins UP Ia, UP Ib and UP II, Uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kD) and has been shown to be a specific marker of terminal urothelial differentiation (Wu et al., 1993, 1994).Synonyms: UP3a, UPIII, UPK3, Uroplakin III
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基因ID
- 7380
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NCBI登录号
- NP_001161046
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UniProt
- O75631
抗原
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