SYNPO 抗体
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北京 101111
Quick Overview for SYNPO 抗体 (ABIN112223)
抗原
See all SYNPO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.
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交叉反应 （详细）
- Species reactivity (tested):Human, Bovine, Rat, Mouse, Guinea Pig and Gerbil. Negative with Rabbit, Frog and Chicken.
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纯化方法
- Affinity chromatography on Protein A
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免疫原
- Isolated Rat kidney glomeruli.
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亚型
- IgG1
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anti-Synaptopodin (SYNPO) (Center) antibody
KO Validated SYNPO 适用: 人, 小鼠, 大鼠 WB, IHC, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Synaptopodin (SYNPO) (AA 97-300) antibodySYNPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Synaptopodin (SYNPO) (Center) antibodyKO Validated SYNPO 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IHC (p) 宿主: 豚鼠 Polyclonal unconjugated
anti-Synaptopodin (SYNPO) (AA 601-700) antibodySYNPO 适用: 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Synaptopodin (SYNPO) (AA 601-700) antibody (FITC)SYNPO 适用: 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal FITC
anti-Synaptopodin (SYNPO) (C-Term) antibodySYNPO 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Synaptopodin (SYNPO) (AA 1-260) antibodySYNPO 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunofluorescence. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue: 1/5-1/10 for 1 h at RT (Aftermicrowave treatment). Immunoblotting (Western): 0.1-0.5 μg/mL. The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL distilled water
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缓冲液
- Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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: "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis." in: Cells, Vol. 9, Issue 2, (2021) (PubMed).
: "Phosphorylation of ribosomal protein S6 mediates compensatory renal hypertrophy." in: Kidney international, Vol. 87, Issue 3, pp. 543-56, (2015) (PubMed).
: "The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation." in: Human molecular genetics, Vol. 19, Issue 23, pp. 4663-76, (2010) (PubMed).
: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).
: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).
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: "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis." in: Cells, Vol. 9, Issue 2, (2021) (PubMed).
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- SYNPO (Synaptopodin (SYNPO))
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别名
- Synaptopodin / SYNPO
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背景
- Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).Synonyms: KIAA1029
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基因ID
- 11346, 9606
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UniProt
- Q8N3V7
抗原
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