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SYNPO 抗体

This anti-SYNPO antibody is a 小鼠 单克隆 antibody detecting SYNPO in WB, IF, IHC (p) 和 IHC (fro). Suitable for 人, 小鼠, 大鼠, Cow, Gerbil 和 豚鼠. This Primary Antibody has been cited in 5+ publications.
产品编号 ABIN112223
发货至: 中国

Quick Overview for SYNPO 抗体 (ABIN112223)

抗原

See all SYNPO 抗体
SYNPO (Synaptopodin (SYNPO))

适用

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人, 小鼠, 大鼠, Cow, Gerbil, 豚鼠

宿主

  • 49
  • 5
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小鼠

克隆类型

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单克隆

标记

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This SYNPO antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

G1D4
  • 特异性

    The antibody reacts specifically with Synaptopodin. It recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-Actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation dependent expression during postnatal maturation of Rat brain. Differentiation dependent expression in cultured hippocampal neurons.

    交叉反应 (详细)

    Species reactivity (tested):Human, Bovine, Rat, Mouse, Guinea Pig and Gerbil. Negative with Rabbit, Frog and Chicken.

    纯化方法

    Affinity chromatography on Protein A

    免疫原

    Isolated Rat kidney glomeruli.

    亚型

    IgG1
  • 应用备注

    Immunofluorescence. Immunohistochemistry on Frozen SectionsImmunohistochemistry on Paraffin Embedded Tissue: 1/5-1/10 for 1 h at RT (Aftermicrowave treatment). Immunoblotting (Western): 0.1-0.5 μg/mL. The antibody also reacts with a 44 kD degradation fragment of Synaptopodin.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 1 mL distilled water

    缓冲液

    Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Tan, Rak-Raszewska, Skovorodkin, Vainio: "Mouse Embryonic Stem Cell-Derived Ureteric Bud Progenitors Induce Nephrogenesis." in: Cells, Vol. 9, Issue 2, (2021) (PubMed).

    Xu, Chen, Dong, Meyuhas, Chen: "Phosphorylation of ribosomal protein S6 mediates compensatory renal hypertrophy." in: Kidney international, Vol. 87, Issue 3, pp. 543-56, (2015) (PubMed).

    Yates, Papakrivopoulou, Long, Goggolidou, Connolly, Woolf, Dean: "The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation." in: Human molecular genetics, Vol. 19, Issue 23, pp. 4663-76, (2010) (PubMed).

    Câmpean, Karpe, Haas, Atalla, Peters, Rupprecht, Liebner, Acker, Plate, Amann: "Angiopoietin 1 and 2 gene and protein expression is differentially regulated in acute anti-Thy1.1 glomerulonephritis." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1174-84, (2008) (PubMed).

    Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).

  • 抗原

    SYNPO (Synaptopodin (SYNPO))

    别名

    Synaptopodin / SYNPO

    背景

    Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).Synonyms: KIAA1029

    基因ID

    11346, 9606

    UniProt

    Q8N3V7
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