CD8 抗体
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- 抗原 See all CD8 抗体
- CD8
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD8 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody detcts T-cell subpopulation, thmocytes. Antigen distribution: Isolated cells: Subpopulation of T-cells in the peripheral blood. Tissue sections: Rib 6/1 detects the T-cell regions in lymphoid organs and stains cortical and medullary thymocytes positively. Monoclonal antibody Rib 6/1 is used for the identificaton of CD8 bearing cells, mainly Tcytotoxic and suppressor lymphocytes, in tissue sections and isolated cell suspensions. Preliminary results indicate that the Rib 6/1 antigen is the β-chain of the CD8 antigen which is not expressed by NK-cells.
- 纯化方法
- Affinity chromatography
- 免疫原
- Rat lymphoblasts
- 克隆位点
- Rib 6-1
- 亚型
- IgG1
- Top Product
- Discover our top product CD8 Primary Antibody
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anti-CD8 (CD8) (AA 51-150) antibody
CD8 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) antibody (PE)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PE
anti-CD8 (CD8) (AA 22-187) antibodyCD8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) antibodyCD8 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 3C3 unconjugated Recombinant Antibody
anti-CD8 (CD8) antibodyCD8 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-31 unconjugated
anti-CD8 (CD8) antibody (Biotin)CD8 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-31 Biotin
anti-CD8 (CD8) antibody (FITC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 FITC
anti-CD8 (CD8) antibody (PE)CD8 适用: Cat FACS 宿主: 小鼠 Monoclonal fCD8 PE
anti-CD8 (CD8) antibody (PerCP)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PerCP
anti-CD8 (CD8) antibody (APC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 APC
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- 应用备注
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Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: rat spleen. Does not react on routinely processed paraffin sections. Flow cytometry (use 10 μL of neat antibody to label 10^6 cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30 min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L)minimal-cross reaction to rat) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water.
- 缓冲液
- Phosphate buffered saline pH 7.2 (PBS), 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C/-80 °C
- 储存方法
- Store (in aliquots) at -20 °C to -80 °C.
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- 抗原
- CD8
- 别名
- CD8 (CD8 产品)
- 背景
- The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
- 基因ID
- 24930
- NCBI登录号
- NP_113726
- UniProt
- P07725
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