MHC Class II (I-A K,s,r) 抗体
This anti- antibody is a 大鼠 单克隆 antibody detecting in FACS 和 IHC (fro). Suitable for 小鼠.
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Quick Overview for MHC Class II (I-A K,s,r) 抗体 (ABIN111990)
抗原
MHC Class II (I-A K,s,r)
适用
小鼠
宿主
大鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
ER-TR2
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纯化方法
- Affinity Chromatography
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免疫原
- Murine thymic reticulum Remarks: MHC Class II antigens are heterodimers consisting of one alpha-chain (31-34kDa) and one beta-chain (26-29kDa). The epitope has not been further characterized.
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亚型
- IgG2b
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应用备注
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Immunohistochemistry on frozen sections (1 μg/mL, 1: 200). Suggested positive control: Mouse spleen. FACS. Does not react on routinely processed paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase
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限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water (=stock solution).
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浓度
- 0.2 mg/mL
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缓冲液
- Phosphate buffered saline, pH 7.2 (PBS), 0.01 % Thimerosal, 10 mg/mL BSA
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2 - 8 °C.
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- MHC Class II (I-A K,s,r)
抗原
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