Transferrin Receptor 抗体 (Biotin)
Quick Overview for Transferrin Receptor 抗体 (Biotin) (ABIN111981)
抗原
See all Transferrin Receptor (TFRC) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- ER-MP21 stains proliferating cells of all types as well as cells taking up iron for special needs, such as late erythroid precursors and some mature macrophages. The antibody inhibits the iron uptake and the proliferation of macrophage precursors from bone marrow and early macrophage precursor cell lines. ER-MP21 recognizes the transferrin receptor but does not compete with transferrin binding. The antigen is a 200 kDa protein (non-reducing conditions) consisting of two identical chains. The recognized epitope of the receptor is not located at the transferrin binding site. Antigen Distribution: Macrophage progeny at day 7 in bone marrow cultures, treated in parallel on various days by irradiation (1500 rad 60Co γ-radiation) or addition of ER-MP21 (30 μg/mL final concentration). Cell numbers were quantified using the MTT assay and expressed relative to untreated controls. Relative growth inhibition calculated from data represented in left figure. For mAbor radiation treated cultures, the number of cell cycles needed to reach the cell number in untreated controls was calculated. Values obtained for irradiated cultures were taken as 100 % inhibition controls and values for ER-MP21 - treated cultures were expressed relative to these (See Figure. 1).
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交叉反应 （详细）
- Species reactivity (tested):Mouse: Transferrin Receptor.
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纯化方法
- Affinity Chromatography
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免疫原
- Macrophages precursor cells.
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亚型
- IgG2a
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anti-Transferrin Receptor (p90, CD71) (TFRC) antibody
TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 578-765) antibodyTFRC 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibodyTFRC 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-140) antibodyTFRC 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) antibodyTFRC 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibody (Biotin)TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 Biotin
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 608-727) antibodyTFRC 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 小鼠 Monoclonal 1A1B2 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 621-720) antibodyTFRC 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 567-744) antibodyTFRC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 572-757) antibodyTFRC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry (5 μg): Use 5 μL of the suggested dilution to label 10^6 cells. Immunohistochemistry on Frozen Sections: 2 μg/mL (1/200). Does not react on routinely processed Paraffin Sections. Suggested positive control: Mouse spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 10 mg/mL BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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别名
- CD71 / TFRC
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背景
- The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.Synonyms: TfR1, Transferrin receptor protein 1, p90
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基因ID
- 22042
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NCBI登录号
- NP_035768
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UniProt
- Q62351
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途径
- Transition Metal Ion Homeostasis
抗原
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