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SFTPD 抗体 (Conformational Domain) (Biotin)

Cited in 1 publication. This anti-SFTPD antibody is a 小鼠 单克隆 antibody detecting SFTPD in WB, IHC (p) 和 IHC (fro). Suitable for 人 和 大鼠.
产品编号 ABIN111975
发货至: 中国
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Quick Overview for SFTPD 抗体 (Conformational Domain) (Biotin) (ABIN111975)

抗原

See all SFTPD 抗体
SFTPD (Surfactant Protein D (SFTPD))

适用

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人, 大鼠

宿主

  • 51
  • 23
小鼠

克隆类型

  • 50
  • 24
单克隆

标记

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  • 1
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This SFTPD antibody is conjugated to Biotin

应用范围

  • 59
  • 25
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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

IIE11
  • 抗原表位

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    Conformational Domain

    特异性

    II E11 recognizes a conformational epitope of SP-D. No cross reactivity with rat SP-A. Antigen distribution: Tissue sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.

    免疫原

    Purified rat surfactant protein D from bronchoalveolar lavage

    亚型

    IgG2b
  • 应用备注

    Immunohistochemistry on frozen sections (1 μg/mL, 1: 400). Immunohistochemistry on paraffin sections (1: 100 freshly prepared for staining rat tissues. Fixation: Acetone 5 min 4 C, paraformaldehyde / paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W). Western Blot. Suggested positive control: Rat lung.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore by adding 0.5 mL distilled water.

    浓度

    0.4 mg/mL

    缓冲液

    Phosphate buffered saline pH 7.2, (PBS), 5 mg/mL bovine serum albumin (BSA) as a stabilizer, no preservative.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store (in aliquots) at -20 °C for longer.
  • Bräuer, Kindler, Jäger, Sel, Nölle, Pleyer, Ochs, Paulsen: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." in: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).

  • 抗原

    SFTPD (Surfactant Protein D (SFTPD))

    别名

    SFTPD

    背景

    Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

    基因ID

    25350

    NCBI登录号

    NP_037010

    UniProt

    P35248
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