SFTPD 抗体 (Conformational Domain) (Biotin)
Quick Overview for SFTPD 抗体 (Conformational Domain) (Biotin) (ABIN111975)
抗原
See all SFTPD 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Conformational Domain
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特异性
- II E11 recognizes a conformational epitope of SP-D. No cross reactivity with rat SP-A. Antigen distribution: Tissue sections: alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.
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免疫原
- Purified rat surfactant protein D from bronchoalveolar lavage
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亚型
- IgG2b
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应用备注
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Immunohistochemistry on frozen sections (1 μg/mL, 1: 400). Immunohistochemistry on paraffin sections (1: 100 freshly prepared for staining rat tissues. Fixation: Acetone 5 min 4 C, paraformaldehyde / paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W). Western Blot. Suggested positive control: Rat lung.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore by adding 0.5 mL distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Phosphate buffered saline pH 7.2, (PBS), 5 mg/mL bovine serum albumin (BSA) as a stabilizer, no preservative.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store (in aliquots) at -20 °C for longer.
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: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." in: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).
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: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." in: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).
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- SFTPD (Surfactant Protein D (SFTPD))
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别名
- SFTPD
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背景
- Surfactant protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D
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基因ID
- 25350
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NCBI登录号
- NP_037010
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UniProt
- P35248
抗原
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