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SFTPD 抗体 (Conformational Domain)

There is 1 publication for this product available. The 小鼠 单克隆 anti-SFTPD antibody is suitable to detect SFTPD in samples from 人 和 大鼠. It has been validated for WB, IHC (fro) 和 EIA.
产品编号 ABIN111974
发货至: 中国
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Quick Overview for SFTPD 抗体 (Conformational Domain) (ABIN111974)

抗原

See all SFTPD 抗体
SFTPD (Surfactant Protein D (SFTPD))

适用

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人, 大鼠

宿主

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小鼠

克隆类型

  • 50
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单克隆

标记

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This SFTPD antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

克隆位点

IIE11 (SPDE)
  • 抗原表位

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    Conformational Domain

    特异性

    SPDE recognizes a conformational epitope of SP-D. Clone SPDE is a reclone from clone IIE11, with identical reactivity. Antigen Distribution on Tissue Sections: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on rat skin, colon, kidney and liver.

    纯化方法

    Affinity Chromatography.

    免疫原

    Purified Rat Surfactant protein D from bronchoalveolar lavage.

    亚型

    IgG2b
  • 应用备注

    Immunohistochemistry on Frozen Sections: 2 μg/mL (1/250)Does not react on routinely processed paraffin sections. Recommneded Positive Control: Rat lung. Has been described to work in Western blots.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore by adding 0.5 mL distilled water (= 0.5 mg/mL Stock Solution).

    浓度

    0.5 mg/mL

    缓冲液

    Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 2-8 °C for up to one month. For long term storage, aliquot and freeze unused portion at -20 °C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Avoid prolonged exposure to light.
  • Bräuer, Kindler, Jäger, Sel, Nölle, Pleyer, Ochs, Paulsen: "Detection of surfactant proteins A and D in human tear fluid and the human lacrimal system." in: Investigative ophthalmology & visual science, Vol. 48, Issue 9, pp. 3945-53, (2007) (PubMed).

  • 抗原

    SFTPD (Surfactant Protein D (SFTPD))

    别名

    SFTPD

    背景

    Surfactant Protein D (SP-D) is a Ca2+-dependent carbohydrate-binding protein and is structurally similar to other C-type mammalian lectins, such as conglutinin and SP-A. It has a molecular size of approximately 43 kDa in its reduced state, 620 kDa in non-dissociating conditions. SP-D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D

    基因ID

    6441

    NCBI登录号

    NP_003010

    UniProt

    P35247
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