Mucin-Like Carcinoma Antigen (MCA) 抗体
The 小鼠 单克隆 anti-Mucin-Like Carcinoma Antigen (MCA) antibody (Clone b-12) (ABIN111966) specifically detects Mucin-Like Carcinoma Antigen (MCA) in IHC (fro) 和 IHC (p).
The antibody is reactive with 人 samples.
1 image
产品编号 ABIN111966
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Mucin-Like Carcinoma Antigen (MCA) 抗体 (ABIN111966)
抗原
Mucin-Like Carcinoma Antigen (MCA)
适用
人
宿主
小鼠
克隆类型
单克隆
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
B-12
-
-
纯化方法
- Affinity Chromatography.
-
免疫原
- Breast carcinoma cell lines.
-
亚型
- IgG1
-
-
-
应用备注
-
Immunohistochemistry on: Frozen sections: 0.5 μg/mL (1/200). Paraffin sections: 4 μg/mL (1/25), Proteinase K pretreatment for antigen retrievalrecommended. Suggested positive control: Human uterus. Antigen Distribution: In contrast to MCA producing tumors, the b-12 related antigen is onlylocated at the MCA producing sites such as glandular cell surfaces or glandular tubuli. InMCA producing tumors, where cells become disorganized, the b-12 antigen is secreted intostromal tissue and blood vessels. (See Table 1. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7 min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.
-
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 0.5 mL distilled water.
-
浓度
- 0.1 mg/mL
-
缓冲液
- PBS, buffer pH 7.2 with 0.05 % Sodium Azide as preservative and 2 mg/mL BSA as stabilizer
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- Mucin-Like Carcinoma Antigen (MCA)
-
背景
- Synonyms: Marker for Mucin Producing Cells
抗原
-
You are here:
