Endothelial Cells 抗体
This anti-Endothelial Cells antibody (ABIN111945) is a Rat Monoclonal antibody detecting Endothelial Cells in IHC (fro). Suitable for Human.
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Quick Overview for Endothelial Cells 抗体 (ABIN111945)
抗原
Endothelial Cells
适用
人
宿主
大鼠
克隆类型
单克隆
标记
This Endothelial Cells antibody is un-conjugated
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
1F10
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纯化方法
- Affinity A chromatography.
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免疫原
- Cultured HUVECs.
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亚型
- IgG2a
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anti-Endothelial Cells antibody
适用: Pig IHC (fro), EIA 宿主: 小鼠 Monoclonal PE1 unconjugated
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应用备注
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Immunohistochemistry on Frozen sections: 2 μg/mL (1: 100),Suggested positive control: Human placenta. Antigen Distribution: The antigen is found in variable amounts on freshly isolated isolated HUVE cells. Antigenexpression on cultured HUVEC is dependent on the culture conditions. Best results wereobtained (40-70 % pos. ) by daily renewal of FCS containing medium supplemented withendothelial cell conditioned supernatant whereas commercial available specificendothelial cell media have no influence on the antigen expression (see Table 1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.2 mg/mL
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缓冲液
- PBS, pH 7.2 containing 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use
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储存条件
- 4 °C
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储存方法
- Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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- Endothelial Cells
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背景
- Synonyms: Endothelial Cell marker
抗原
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