CD9 抗体
Quick Overview for CD9 抗体 (ABIN111919)
抗原
See all CD9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Clone K41 detects CD9. Antigen distribution on Isolated cells: CD9 is mainly expressed on platelets, on early B-cells, and monocytes. Further reactivity could be seen with basophiles and eosinophiles. CD9 is used for the characterization of leukaemias and lymphomas. 90 % of non-T acute lymphoblastic leukaemic cells, 50 % of acute myeloid as well as chronic lymphoid leukaemias express the antigen. Tissue sections: glomeruli, sinus histiocytes, germinal centre, brain white matter, nerves, skin, and vascular smooth muscles.
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纯化方法
- Affinity chromatography.
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免疫原
- Vero cells
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亚型
- IgG1
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anti-CD9 (CD9) (AA 112-195) antibody
CD9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5A6D8 unconjugated
anti-CD9 (CD9) (AA 112-195) antibodyCD9 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E8H1 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CD9-1619 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal CD9-2343 unconjugated
anti-CD9 (CD9) (Internal Region) antibodyCD9 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 5G6 unconjugated
anti-CD9 (CD9) (AA 101-200) antibodyCD9 适用: 人, 小鼠, Cow, 大鼠, Pig FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibody (Biotin)CD9 适用: 小鼠 FACS, WB, IP, ICC 宿主: 大鼠 Monoclonal EM-04 Biotin
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, WB, IHC (p), MS 宿主: 小鼠 Monoclonal MEM-61 unconjugated
anti-CD9 (CD9) (AA 115-145) antibodyCD9 适用: 人 FACS, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for Immunohistochemistry on: Frozen sections: 2-5 μg/mL, 1/80-1/200). Paraffin sections: 8 μg/mL, 1/50, pretreatment is not necessary. Suggested positive control: human tonsil. Also works in FACS and Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum
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限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % thimerosal as a preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD9
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别名
- CD9
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背景
- CD9 is a member of the tetraspan or transmembrane 4 superfamily. The family members (CD9, CD63, CD37, Cd53, CD81, CD82, CD151) show a high degree of homology in their membrane-spanning domain. CD9 is the only member with N-linked sugar bound to the first extracellular segment. Tetraspans are expressed by many cell types and show a different subcellular distribution. CD9 is constitutively surface-expressed by platelets and pre-B cells and is present in the alpha-granules of platelets. The participation of different tetraspan members in signalling complexes on B and T cells have been described. Furthermore, association of CD9 with beta1 integrins has been found. Anti CD9 monoclonal antibodies induce homotypic pre-B cell aggregation and fibronectin receptor-mediated adherence of these cells to bone marrow fibroblasts. Recent observations suggest a role of CD9 in adhesion.Synonyms: 5H9 antigen, Cell growth-inhibiting gene 2 protein, GIG2, Leukocyte antigen MIC3, MIC3, Motility-related protein, TSPAN29, Tetraspanin-29, p24
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基因ID
- 928
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NCBI登录号
- NP_001760
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UniProt
- P21926
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途径
- Response to Water Deprivation, Cell-Cell Junction Organization
抗原
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